Narishige Pc10 Manual New

– Recommended for teaching & basic patch-clamp; not for advanced research.

Includes a transparent cover to shield the glass capillaries from air currents during the heating process. NARISHIGE WEB NEWS Typical Settings for Common Pipettes Settings vary by glass type, but general rules apply: NARISHIGE WEB NEWS High Heater Value: Produces long, thin pipettes. Low Heater Value: Produces short, thick pipettes. Double Pull: narishige pc10 manual new

: Includes two light (approx. 25g) and two heavy (approx. 100g) weights for fine-tuning tension. – Recommended for teaching & basic patch-clamp; not

: Press the START button to begin the automated pull. Protocol Tips for Common Needs Desired Pipette Type Suggested Settings (Reference Only) Long, Thin Injection Needles High heating value + One-stage pulling Firm Patch-Clamp Electrodes Low heating value + Two-stage pulling Flexible, Sharp Tips Use all available weights to increase pull speed Gradual, Parallel Taper Use fewer weights (e.g., 2 Type Light) Maintenance and Troubleshooting NARISHIGE WEB NEWS Setting Values in the PC-10 Puller - NARISHIGE WEB NEWS Low Heater Value: Produces short, thick pipettes

The PC-10 allows for single-stage or two-stage pulling. The standard method for general-purpose intracellular electrodes is usually a single stage, while patch-clamp electrodes often benefit from a two-stage approach.