The initial bioprocess for mAb-A production involved a traditional approach:
The remaining HCPs and DNA carry a negative charge at pH 8.0. Mab-X, with a pI of 8.5, flows through a Q Sepharose FF column. This step reduces HCP to <30 ppm and DNA to <1 pg/mg. A Mab A Case Study In Bioprocess Development
Focuses on cell culture optimization, including host cell line characterization and risk assessments for process parameters such as pH, dissolved oxygen, and initial cell density. Downstream Recovery and Purification: The initial bioprocess for mAb-A production involved a
We moved from a conventional stirred-tank model to implementing a single-use bioreactor (SUB) strategy for the clinical launch. with a pI of 8.5